plasmids containing the target gene Search Results


atcc accession number  (ATCC)
90
ATCC atcc accession number
Atcc Accession Number, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/us08754199-69-53-53?v=ATCC
Average 90 stars, based on 1 article reviews
atcc accession number - by Bioz Stars, 2026-08
90/100 stars
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pci-neo plasmid containing the neomycin phosphotransferase gene  (Promega)
90
Promega pci-neo plasmid containing the neomycin phosphotransferase gene
Pci Neo Plasmid Containing The Neomycin Phosphotransferase Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pm16785533-99-9-16?v=Promega
Average 90 stars, based on 1 article reviews
pci-neo plasmid containing the neomycin phosphotransferase gene - by Bioz Stars, 2026-08
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plasmid containing a renilla luciferase reporter gene under the tk promoter  (Amura Ltd)
90
Amura Ltd plasmid containing a renilla luciferase reporter gene under the tk promoter
Plasmid Containing A Renilla Luciferase Reporter Gene Under The Tk Promoter, supplied by Amura Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc03405836-88-7-24?v=Amura+Ltd
Average 90 stars, based on 1 article reviews
plasmid containing a renilla luciferase reporter gene under the tk promoter - by Bioz Stars, 2026-08
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a plasmid containing the ci -βγ-crystallin gene, codon-optimized for escherichia coli  (Blue Heron Biotech)
90
Blue Heron Biotech a plasmid containing the ci -βγ-crystallin gene, codon-optimized for escherichia coli
A Plasmid Containing The Ci βγ Crystallin Gene, Codon Optimized For Escherichia Coli, supplied by Blue Heron Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pm27992995-53-7-14?v=Blue+Heron+Biotech
Average 90 stars, based on 1 article reviews
a plasmid containing the ci -βγ-crystallin gene, codon-optimized for escherichia coli - by Bioz Stars, 2026-08
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reporter plasmid containing the firefly luciferase gene under the control of multiple camp-responsive elements (cres)  (Promega)
90
Promega reporter plasmid containing the firefly luciferase gene under the control of multiple camp-responsive elements (cres)
Reporter Plasmid Containing The Firefly Luciferase Gene Under The Control Of Multiple Camp Responsive Elements (Cres), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc11514929-41-27-35?v=Promega
Average 90 stars, based on 1 article reviews
reporter plasmid containing the firefly luciferase gene under the control of multiple camp-responsive elements (cres) - by Bioz Stars, 2026-08
90/100 stars
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p. vivax 18s rrna plasmid dna mra-178  (BEI Resources)
90
BEI Resources p. vivax 18s rrna plasmid dna mra-178
SHERLOCK reaction workflow. (A) DNA extracted from a patient with malaria or infected mosquito is subjected to (B) RPA including T7 promoter-tagged primers for amplification of the Plasmodium 18S rRNA or P. <t>falciparum</t> dhps genes, followed by (C) IVT and LwCas13a:crRNA complex binding to genus-, species-, or genotype-specific target RNA. This binding triggers collateral (D) activation of LwCas13a RNAse activity and cleavage of reporter RNA, separating the fluorescent reporter from its quencher and producing a signal. Abbreviations: RPA, recombinase polymerase amplification; IVT, in vitro transcription; R, reporter; Q, quencher.
P. Vivax 18s Rrna Plasmid Dna Mra 178, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc08213918-101-27-56?v=BEI+Resources
Average 90 stars, based on 1 article reviews
p. vivax 18s rrna plasmid dna mra-178 - by Bioz Stars, 2026-08
90/100 stars
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plasmid pcaggs containing the sars-related coronavirus 2; wuhan-hu-1 spike glycoprotein gene (soluble, stabilized)  (BEI Resources)
90
BEI Resources plasmid pcaggs containing the sars-related coronavirus 2; wuhan-hu-1 spike glycoprotein gene (soluble, stabilized)
SHERLOCK reaction workflow. (A) DNA extracted from a patient with malaria or infected mosquito is subjected to (B) RPA including T7 promoter-tagged primers for amplification of the Plasmodium 18S rRNA or P. <t>falciparum</t> dhps genes, followed by (C) IVT and LwCas13a:crRNA complex binding to genus-, species-, or genotype-specific target RNA. This binding triggers collateral (D) activation of LwCas13a RNAse activity and cleavage of reporter RNA, separating the fluorescent reporter from its quencher and producing a signal. Abbreviations: RPA, recombinase polymerase amplification; IVT, in vitro transcription; R, reporter; Q, quencher.
Plasmid Pcaggs Containing The Sars Related Coronavirus 2; Wuhan Hu 1 Spike Glycoprotein Gene (Soluble, Stabilized), supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc09928730-374-5-18?v=BEI+Resources
Average 90 stars, based on 1 article reviews
plasmid pcaggs containing the sars-related coronavirus 2; wuhan-hu-1 spike glycoprotein gene (soluble, stabilized) - by Bioz Stars, 2026-08
90/100 stars
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plasmid containing the 18s rrna gene  (BEI Resources)
90
BEI Resources plasmid containing the 18s rrna gene
SHERLOCK reaction workflow. (A) DNA extracted from a patient with malaria or infected mosquito is subjected to (B) RPA including T7 promoter-tagged primers for amplification of the Plasmodium 18S rRNA or P. <t>falciparum</t> dhps genes, followed by (C) IVT and LwCas13a:crRNA complex binding to genus-, species-, or genotype-specific target RNA. This binding triggers collateral (D) activation of LwCas13a RNAse activity and cleavage of reporter RNA, separating the fluorescent reporter from its quencher and producing a signal. Abbreviations: RPA, recombinase polymerase amplification; IVT, in vitro transcription; R, reporter; Q, quencher.
Plasmid Containing The 18s Rrna Gene, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pm39075150-218-5-16?v=BEI+Resources
Average 90 stars, based on 1 article reviews
plasmid containing the 18s rrna gene - by Bioz Stars, 2026-08
90/100 stars
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clostron plasmid targeted to the hall a- hyper arcb gene (clc2465)  (ATUM Bio)
90
ATUM Bio clostron plasmid targeted to the hall a- hyper arcb gene (clc2465)
Plasmids and primers used in this study
Clostron Plasmid Targeted To The Hall A Hyper Arcb Gene (Clc2465), supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc08386421-0-3-15?v=ATUM+Bio
Average 90 stars, based on 1 article reviews
clostron plasmid targeted to the hall a- hyper arcb gene (clc2465) - by Bioz Stars, 2026-08
90/100 stars
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plasmid containing cloned target sequences hadv hexon gene  (RayBiotech inc)
90
RayBiotech inc plasmid containing cloned target sequences hadv hexon gene
Plasmids and primers used in this study
Plasmid Containing Cloned Target Sequences Hadv Hexon Gene, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc09715001-120-22-27?v=RayBiotech+inc
Average 90 stars, based on 1 article reviews
plasmid containing cloned target sequences hadv hexon gene - by Bioz Stars, 2026-08
90/100 stars
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plasmids containing the gene-of-interest  (GenScript corporation)
90
GenScript corporation plasmids containing the gene-of-interest
Plasmids and primers used in this study
Plasmids Containing The Gene Of Interest, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc12077596-614-19-20?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
plasmids containing the gene-of-interest - by Bioz Stars, 2026-08
90/100 stars
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plasmid for his-tagged t7 rna polymerase  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare plasmid for his-tagged t7 rna polymerase
Plasmids and primers used in this study
Plasmid For His Tagged T7 Rna Polymerase, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+containing+the+target+gene/pmc08111789-1585-41-33?v=Johns+Hopkins+HealthCare
Average 90 stars, based on 1 article reviews
plasmid for his-tagged t7 rna polymerase - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


SHERLOCK reaction workflow. (A) DNA extracted from a patient with malaria or infected mosquito is subjected to (B) RPA including T7 promoter-tagged primers for amplification of the Plasmodium 18S rRNA or P. falciparum dhps genes, followed by (C) IVT and LwCas13a:crRNA complex binding to genus-, species-, or genotype-specific target RNA. This binding triggers collateral (D) activation of LwCas13a RNAse activity and cleavage of reporter RNA, separating the fluorescent reporter from its quencher and producing a signal. Abbreviations: RPA, recombinase polymerase amplification; IVT, in vitro transcription; R, reporter; Q, quencher.

Journal: EBioMedicine

Article Title: A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

doi: 10.1016/j.ebiom.2021.103415

Figure Lengend Snippet: SHERLOCK reaction workflow. (A) DNA extracted from a patient with malaria or infected mosquito is subjected to (B) RPA including T7 promoter-tagged primers for amplification of the Plasmodium 18S rRNA or P. falciparum dhps genes, followed by (C) IVT and LwCas13a:crRNA complex binding to genus-, species-, or genotype-specific target RNA. This binding triggers collateral (D) activation of LwCas13a RNAse activity and cleavage of reporter RNA, separating the fluorescent reporter from its quencher and producing a signal. Abbreviations: RPA, recombinase polymerase amplification; IVT, in vitro transcription; R, reporter; Q, quencher.

Article Snippet: To determine the analytical sensitivity of the SHERLOCK assays, we performed each assay in triplicate using serially diluted template DNA from P. falciparum strain Dd2 (MRA-150G) for P. falciparum, P. vivax 18S rRNA plasmid DNA (MRA-178), P. ovale 18S rRNA plasmid DNA (MRA-180), P. malariae 18S rRNA plasmid DNA (MRA-179), and P. knowlesi genomic DNA (MRA-456G) (Bei Resources, Manassas, VA).

Techniques: Infection, Amplification, Binding Assay, Activation Assay, Activity Assay, Recombinase Polymerase Amplification, In Vitro

Analytical sensitivity of Plasmodium spp. SHERLOCK assays. (a) Pan- Plasmodium assay vs. P. falciparum strain 3D7 gDNA (b) Pan- Plasmodium assay vs. P. vivax 18S rRNA plasmid DNA (c) P. falciparum assay vs. P. falciparum strain 3D7 gDNA (d) P. vivax assay vs. P. vivax 18S rRNA plasmid DNA. N = 3 technical replicates, n = 1 biological replicate. Unpaired student's T test vs. NTC, ± SEM. Abbreviations: NTC, no template control.

Journal: EBioMedicine

Article Title: A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

doi: 10.1016/j.ebiom.2021.103415

Figure Lengend Snippet: Analytical sensitivity of Plasmodium spp. SHERLOCK assays. (a) Pan- Plasmodium assay vs. P. falciparum strain 3D7 gDNA (b) Pan- Plasmodium assay vs. P. vivax 18S rRNA plasmid DNA (c) P. falciparum assay vs. P. falciparum strain 3D7 gDNA (d) P. vivax assay vs. P. vivax 18S rRNA plasmid DNA. N = 3 technical replicates, n = 1 biological replicate. Unpaired student's T test vs. NTC, ± SEM. Abbreviations: NTC, no template control.

Article Snippet: To determine the analytical sensitivity of the SHERLOCK assays, we performed each assay in triplicate using serially diluted template DNA from P. falciparum strain Dd2 (MRA-150G) for P. falciparum, P. vivax 18S rRNA plasmid DNA (MRA-178), P. ovale 18S rRNA plasmid DNA (MRA-180), P. malariae 18S rRNA plasmid DNA (MRA-179), and P. knowlesi genomic DNA (MRA-456G) (Bei Resources, Manassas, VA).

Techniques: Plasmid Preparation, Control

95% lower limits of detection (LOD) for Plasmodium spp. SHERLOCK assays. Input is 1 µL of DNA template per reaction.

Journal: EBioMedicine

Article Title: A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

doi: 10.1016/j.ebiom.2021.103415

Figure Lengend Snippet: 95% lower limits of detection (LOD) for Plasmodium spp. SHERLOCK assays. Input is 1 µL of DNA template per reaction.

Article Snippet: To determine the analytical sensitivity of the SHERLOCK assays, we performed each assay in triplicate using serially diluted template DNA from P. falciparum strain Dd2 (MRA-150G) for P. falciparum, P. vivax 18S rRNA plasmid DNA (MRA-178), P. ovale 18S rRNA plasmid DNA (MRA-180), P. malariae 18S rRNA plasmid DNA (MRA-179), and P. knowlesi genomic DNA (MRA-456G) (Bei Resources, Manassas, VA).

Techniques:

(a) Pan- Plasmodium SHERLOCK assay can detect all five Plasmodium species in humans, while (b) P. falciparum , and (c) P. vivax SHERLOCK assays can differentiate between Plasmodium species. Input for all species was the equivalent of 100,000 parasites/µL as either species-specific 18S plasmid or gDNA n ( P. knowlesi only). N = 3 technical replicates, n = 1 biological replicate. Unpaired student's T test vs. NTC, ± SEM. Abbreviations: NTC, no template control.

Journal: EBioMedicine

Article Title: A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

doi: 10.1016/j.ebiom.2021.103415

Figure Lengend Snippet: (a) Pan- Plasmodium SHERLOCK assay can detect all five Plasmodium species in humans, while (b) P. falciparum , and (c) P. vivax SHERLOCK assays can differentiate between Plasmodium species. Input for all species was the equivalent of 100,000 parasites/µL as either species-specific 18S plasmid or gDNA n ( P. knowlesi only). N = 3 technical replicates, n = 1 biological replicate. Unpaired student's T test vs. NTC, ± SEM. Abbreviations: NTC, no template control.

Article Snippet: To determine the analytical sensitivity of the SHERLOCK assays, we performed each assay in triplicate using serially diluted template DNA from P. falciparum strain Dd2 (MRA-150G) for P. falciparum, P. vivax 18S rRNA plasmid DNA (MRA-178), P. ovale 18S rRNA plasmid DNA (MRA-180), P. malariae 18S rRNA plasmid DNA (MRA-179), and P. knowlesi genomic DNA (MRA-456G) (Bei Resources, Manassas, VA).

Techniques: Plasmid Preparation, Control

(a) Pan- Plasmodium, (b) P. falciparum , and (c) P. vivax SHERLOCK assays successfully detected and differentiated Plasmodium infections in diverse clinical isolates. (d) P. falciparum SHERLOCK assay detected both the oocyst and sporozoite stages of DNA extracted from infected mosquitos. Pf gDNA in the mosquito assay was 100,000 parasites/µL. Parasite densities were determined by qPCR for Pf, Pm, and Po and by microscopy for Pv. N = 3 technical replicates, n = 1 biological replicate. Unpaired student's T test vs. NTC, ± SEM. Abbreviations: Pf, P. falciparum; Pv, P. vivax ; Pm, P. malariae; Po, P. ovale ; NTC, no template control; gDNA, genomic DNA; Sub, subject.

Journal: EBioMedicine

Article Title: A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

doi: 10.1016/j.ebiom.2021.103415

Figure Lengend Snippet: (a) Pan- Plasmodium, (b) P. falciparum , and (c) P. vivax SHERLOCK assays successfully detected and differentiated Plasmodium infections in diverse clinical isolates. (d) P. falciparum SHERLOCK assay detected both the oocyst and sporozoite stages of DNA extracted from infected mosquitos. Pf gDNA in the mosquito assay was 100,000 parasites/µL. Parasite densities were determined by qPCR for Pf, Pm, and Po and by microscopy for Pv. N = 3 technical replicates, n = 1 biological replicate. Unpaired student's T test vs. NTC, ± SEM. Abbreviations: Pf, P. falciparum; Pv, P. vivax ; Pm, P. malariae; Po, P. ovale ; NTC, no template control; gDNA, genomic DNA; Sub, subject.

Article Snippet: To determine the analytical sensitivity of the SHERLOCK assays, we performed each assay in triplicate using serially diluted template DNA from P. falciparum strain Dd2 (MRA-150G) for P. falciparum, P. vivax 18S rRNA plasmid DNA (MRA-178), P. ovale 18S rRNA plasmid DNA (MRA-180), P. malariae 18S rRNA plasmid DNA (MRA-179), and P. knowlesi genomic DNA (MRA-456G) (Bei Resources, Manassas, VA).

Techniques: Infection, Microscopy, Control

The dhps SHERLOCK assay detects P. falciparum parasites with (a) mutant (581G) but not (b) wild-type (A581) alleles. Parasite densities were determined by qPCR. N = 3 technical replicates, n = 1 biological replicate,± SEM. Mutant (Mut) and WT gDNA standard inputs were 100,000 parasites/µL.

Journal: EBioMedicine

Article Title: A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping

doi: 10.1016/j.ebiom.2021.103415

Figure Lengend Snippet: The dhps SHERLOCK assay detects P. falciparum parasites with (a) mutant (581G) but not (b) wild-type (A581) alleles. Parasite densities were determined by qPCR. N = 3 technical replicates, n = 1 biological replicate,± SEM. Mutant (Mut) and WT gDNA standard inputs were 100,000 parasites/µL.

Article Snippet: To determine the analytical sensitivity of the SHERLOCK assays, we performed each assay in triplicate using serially diluted template DNA from P. falciparum strain Dd2 (MRA-150G) for P. falciparum, P. vivax 18S rRNA plasmid DNA (MRA-178), P. ovale 18S rRNA plasmid DNA (MRA-180), P. malariae 18S rRNA plasmid DNA (MRA-179), and P. knowlesi genomic DNA (MRA-456G) (Bei Resources, Manassas, VA).

Techniques: Mutagenesis

Plasmids and primers used in this study

Journal: mSphere

Article Title: Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A- hyper

doi: 10.1128/mSphere.00328-21

Figure Lengend Snippet: Plasmids and primers used in this study

Article Snippet: pMTL0007C-E2: ArcB-156a , ClosTron plasmid targeted to the Hall A- hyper arcB gene (CLC2465) , DNA2.0 (Atum).

Techniques: Sequencing, Plasmid Preparation